|Available in: Rats and Mice
The animal is anesthetized and clipped over the abdominal region. Sterile eye lubricant is placed in both eyes, and the clipped skin area over the abdominal wall is surgically prepared. The animal is placed in dorsal recumbency on a surgery board fitted with a sterile board drape. Analgesic is administered.
The surgical site is draped with a sterile drape. To expose the vas deferens, ventral, midline longitudinal incisions are made to the skin and abdominal wall at the level of the posterior abdomen. Padded retractors are used to increase surgical visibility. A dissecting scope may be required for the remainder of this procedure. The vas deferens on each side is externalized and the ducts are permanently double ligated. The area between the ligatures is excised and the vas deferens is returned to the natural position. The abdominal cavity is flushed with sterile saline.
The abdominal incision is closed with approved closing materials (suture or stainless steel clips), and the surgery sites are treated with an approved disinfectant followed by an alcohol swabbing.
A standard sham vasectomy model is prepared as described above with regards to surgical site preparation, exposure of the vas deferens, wound closing, and surgical site disinfection; however, no ligation or resection.