Unilateral Uretal Obstruction Procedure (UUO)
|Available in: Rats
The animal is anesthetized and clipped over the abdominal region. Sterile eye lubricant is placed in both eyes, and the clipped skin area over the abdomen is surgically prepared. The animal is placed in dorsal recumbency on a surgery board fitted with a sterile board drape. Analgesic is administered.
The surgical site is draped with a sterile drape. To expose the ureter, a ventral, midline, longitudinal incision is made to the skin and abdominal wall from approximately the symphysis pubis to the midpoint of the abdomen. Padded retractors may be used to increase visibility. The left (or right) ureter is located and isolated using blunt dissection. Suture is permanently ligated in two locations (approximately 5-15mm apart) around the ureter. The ureter between the ligated sutures is cut away and removed.
The abdominal incisions are closed with approved closing materials (suture or stainless steel clips), and the surgery sites are treated with an approved disinfectant followed by an alcohol swabbing.
A standard sham unilateral ureteral obstruction model is prepared as described above with regards to surgical site preparation, dissection of the abdomen, exposure of the ureters, closing, and surgical site disinfection; however, no tissue is removed.