Oophorohysterectomy (Ovaries and Uterus Removed)

Available in: Rats and Mice Anesthetics Analgesic
Pre-Operative Procedures Intra-Operative Care Post-Operative Care

Surgical Preparation

The animal is anesthetized and clipped over the abdominal region. Sterile eye lubricant is placed in both eyes, and the clipped skin area over the abdomen is surgically prepared. The animal is placed in dorsal recumbency on a surgery board fitted with a sterile board drape. Analgesic is administered.

Surgical Description

The surgical site is draped with a sterile drape. To expose the uterus, midline, longitudinal incisions are made to the skin and abdominal wall from approximately the symphysis pubis to the midpoint of the abdomen. Padded retractors may be used to increase visibility. The uterine horns are located and exteriorized. The uterine vessels, the collateral supplies to the ovaries and above the collateral supplies to the cervix are double ligated. Vessels between the ligatures are transected, and the vessels of the collateral supply are cut. The ovaries and uterine horn are exteriorized. The cervix is double ligated about midway along its length and is transected between sutures. The uterus and ovaries are removed.

Surgical Closing

The abdominal incisions are closed with approved closing materials (suture or stainless steel clips), and the surgery sites are treated with an approved disinfectant followed by an alcohol swabbing.


A standard sham Oophorohysterectomy model is prepared as described above with regards to surgical site preparation, dissection of the abdomen, exposure of the uterine horns and ovaries, closing, and surgical site disinfection; however, no tissue is removed.