Bile Duct Ligation

Available in: Rats and Mice Anesthetics Analgesic
Pre-Operative Procedures Intra-Operative Care Post-Operative Care

Surgical Preparation

The animal is anesthetized and clipped over the abdominal region. Sterile eye lubricant is placed in both eyes, and the clipped skin area over the abdomen is surgically prepared. The animal is placed in dorsal recumbency on a surgery board fitted with a sterile board drape. Analgesic is administered.

Surgical Description

The surgical site is draped with a sterile drape. To expose the bile duct, midline longitudinal incisions are made to the skin and abdominal wall at the level of the bile duct. Padded retractors are used to increase surgical visibility. A dissecting scope may be required for the remainder of this procedure. Surrounding tissues are gently cleared using blunt dissection to expose the bile duct. The duct is permanently ligated inferior to the tributaries from the liver. The abdominal cavity is flushed with sterile saline.

Surgical Closing

The abdominal incisions are closed with approved closing materials (suture or stainless steel clips), and the surgery sites are treated with an approved disinfectant followed by an alcohol swabbing.


A standard sham bile duct ligation model is prepared as described above with regards to surgical site preparation, dissection of the abdomen, exposure of the bile duct, closing, and surgical site disinfection; however, no ligation occurs.